Overview of the P11 experimental hutch.
The macromolecular crystallography beamline P11 at Petra III is dedicated to diffraction experiments of biological samples. The beamline provides an end-station for crystallography experiment operating between 6 keV and 30 keV. The basic idea for the beamline design is to provide an extremely stable and flexible setup ideally suited for micro- and nano-beam applications.
GENERAL SPECIFICATIONS
|
techniques available |
macromolecular crystallography (imaging of biological samples) |
|---|---|
|
photon source |
undulator |
|
period number of periods max power |
31.4 mm 63 9.5 kW |
|
source brilliance |
1018 ph / s / 0.1% bw / mA |
|
polarization available |
linear horizontal |
|
energy range |
5.5 keV - 30 keV |
|
beamline energy resolution |
1.3 x 10-4 (Si-111) / 0.28 x 10-4 (Si-113) |
|
max flux ON SAMPLE |
1.3 x 1013 ph/s at 12 keV |
|
spot size ON SAMPLE |
4 x 9 μm² up to 200 x 200 μm² (v x h) with full flux;
1 x 1 μm² with > 2 x 1011 ph/s |
|
angle of incidence light – sample |
0° to 60° |
|
sample type |
macromolecular crystals (biological tissue) |
OPTICS
|
type |
double crystal monochromator |
|---|---|
|
grating properties
|
Si-111 and Si-113 reflection |
|
energy range
|
2.4 keV - 30 keV (Si-111) |
|
energy resolution
|
~ 1 eV |
|
type |
1. horizontal focussing mirror |
|---|---|
|
material |
silicon |
|
gracing angle |
2.5 mrad |
|
coatings |
uncoated (2.4 keV - 8 keV) palladium (8 keV - 20 keV) platinum (> 20 keV) |
|
radius of curvature |
-∞ ≤ x ≤ -10 km; ∞ ≥ x ≥ +10 km |
|
type |
2. horizontal focussing mirror |
|---|---|
|
material |
silicon |
|
gracing angle |
2.5 mrad |
|
coatings |
uncoated (2.4 keV - 8 keV) palladium (8 keV - 20 keV) platinum (> 20 keV) |
|
radius of curvature |
-∞ ≤ x ≤ -10 km; ∞ ≥ x ≥ +10 km |
|
type |
vertical focussing mirror |
|---|---|
|
material |
silicon |
|
gracing angle |
2.5 mrad |
|
coatings |
uncoated (2.4 keV - 8 keV) palladium (8 keV - 20 keV) platinum (> 20 keV) |
|
radius of curvature |
100 km ≥ x ≥ 10 km |
ENDSTATION(S)
|
type |
High Precision 1-Axis Goniostat |
|---|---|
|
diffractometer |
inhouse-designed high precision goniometer; sphere of confusion: 100 nm |
|
manipulator |
single air-bearing-based rotation axis equipped with motor-driven crystal centering stages temperature range: 80 K - 350 K transfer system: manual / sample changing robot (uni-puck) control: fully automated |
|
detectors |
Eiger 2X 16M (replacing Pilatus 6M fast) Vortex-EM |
|
sample environment |
temperature range: 80 K - 400 K |
DETECTOR(S)
|
type |
Eiger 2X 16M |
|---|---|
|
count rate |
107 ph/s |
|
spatial resolution |
75 µm |
|
2theta range |
0° - 60° |
|
frame rate |
up to 100 Hz |
|
read-out |
continuous |
|
dead time between exposures |
< 100 ns (max.loss of 1 count/pixel) |
|
pixel size |
75 x 75 µm |
|
energy detection thresholds |
2 |
|
point-spread function |
1 pixel |
|
array size |
4148 x 4362 pixel |
|
field of view |
311.1 x 327.15 mm |
|
degrees of freedom / translation stages |
1 ; detector distance |
|
data format |
HDF5/NeXus |
|
typical collection time |
18 s per data set |
|
type |
Vortex-EM |
|---|---|
|
energy resolution |
100 eV |
|
count rate |
2 x 105 cts/s |
|
2theta range |
fixed 110° |
|
typical collection time |
1 s per energy point |
CONTROL/DATA ANALYSIS
|
Graphical user interface |
CrystalControl (in-house develeopment) |
|---|---|
|
Data output type |
diffraction images, fluorescence spectra |
|
Data output |
hdf5, tif, ascii, (cbf for Pilatus) |
|
software(s) for quantitative analysis |
XDS, XDSAPP (autoprocessing), CCP4, PHENIX, Mosflm (autoprocessing), Albula |
SUPPORT LABORATORY
P11 chemistry lab equipped with Leica 205C stereo microscope, crystal shifter, fume hood, ThermoStat, centrifuge, crystal growing incubator, and harvesting and cryo-cooling tools.
