Unified Data Sheet

Overview of the P11 experimental hutch.

P11 - High-throughput Macromolecular Crystallography beamline

The macromolecular crystallography beamline P11 at Petra III is dedicated to imaging and diffraction experiments of biological samples. The beamline provides two different end-stations: A crystallography experiment operating between 6 keV and 30 keV and an X-ray microscope utilizing tender X-rays between 2.4 and 10 keV. The basic idea for the beamline design is to provide an extremely stable and flexible setup ideally suited for micro- and nano-beam applications.

CONTACT Engineers

GENERAL SPECIFICATIONS

techniques available

macromolecular crystallography (imaging of biological samples)

photon source

undulator

period

number of periods

max power

31.4 mm

63

9.5 kW

source brilliance 

1018 ph / s / 0.1% bw / mA

polarization available

linear horizontal

energy range 

5.5 keV - 30 keV

beamline energy resolution

1.3 x 10-4 (Si-111) / 0.28 x 10-4 (Si-113)

max flux ON SAMPLE

1.3 x 1013 ph/s at 12 keV

spot size ON SAMPLE 

4 x 9 μm² up to 200 x 200 μm²  (v x h)  with full flux;
1 x 1 μwith > 2 x 1011 ph/s

angle of incidence light – sample 

0° to 60°

sample type

macromolecular crystals (biological tissue)

OPTICS

type

  double crystal monochromator

grating properties 

Si-111 and Si-113 reflection

energy range         

2.4 keV - 30 keV (Si-111)

energy resolution

~ 1 eV

type

  1. horizontal focussing mirror

 material

silicon

gracing angle

2.5 mrad

 coatings      

uncoated (2.4 keV - 8 keV)

palladium (8 keV - 20 keV)

platinum (> 20 keV)

 radius of curvature

-∞ ≤ x -10 km;  ∞ x ≥ +10 km

type

  2. horizontal focussing mirror

 material

silicon

gracing angle

2.5 mrad

 coatings      

uncoated (2.4 keV - 8 keV)

palladium (8 keV - 20 keV)

platinum (> 20 keV)

 radius of curvature

-∞ ≤ x -10 km;  ∞ x ≥ +10 km

type

  vertical focussing mirror

 material

silicon

gracing angle

2.5 mrad

 coatings      

uncoated (2.4 keV - 8 keV)

palladium (8 keV - 20 keV)

platinum (> 20 keV)

 radius of curvature

100 km ≥ x ≥ 10 km

ENDSTATION(S)

High Precision 1-Axis Goniostat

diffractometer

inhouse-designed high precision goniometer; sphere of confusion: 100 nm

manipulator

single air-bearing-based rotation axis equipped with motor-driven crystal centering stages

temperature range: 80 K - 350 K

transfer system: manual / sample changing robot (uni-puck)

control: fully automated


detectors

Eiger 2X 16M (replacing Pilatus 6M fast)

Vortex-EM

sample environment

temperature range: 80 K - 400 K

DETECTOR(S)

Area Detector(s)

type

Eiger 2X 16M

count rate

107 ph/s

spatial resolution

75 µm

2theta range 

0° - 60°

frame rate

up to 100 Hz

read-out

continuous

dead time between exposures

< 100 ns (max.loss of 1 count/pixel)

pixel size  

75 x 75 µm

energy detection thresholds

2

point-spread function

1 pixel

array size

4148 x 4362 pixel

field of view 

311.1 x 327.15 mm

degrees of freedom / translation stages

1 ; detector distance

data format

HDF5/NeXus

typical collection time

18 s per data set

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type 

Pilatus 6M fast

count rate 

106 ph/s

spatial resolution 

172 µm

2theta range  

0° - 60°

frame rate

up to 25 Hz

read-out time 

2.6 ms (continuous data collection)

pixel size         

172 x 172 µm

array size 

2463 x 2527 pixel

field of view  

423.6 x 434.6 mm

 degrees of freedom / translation stages

1 ; detector distance

 typical collection time

90 s per data set
Point Detector(s)

type 

Vortex-EM

energy resolution 

100 eV

count rate 

2 x 105 cts/s

2theta range  

fixed 110°

 typical collection time

1 s per energy point

CONTROL/DATA ANALYSIS

Graphical user interface

CrystalControl (in-house develeopment)

Data output type

diffraction images, fluorescence spectra

Data output

hdf5, tif, ascii, (cbf for Pilatus)

software(s) for quantitative analysis

XDS, XDSAPP, CCP4, PHENIX, Mosflm (for automatic data processing); Albula

SUPPORT LAB

wet lab equipped with Leica 205C stereo microscope, Nikon fluorescence microscope with cryo-cooling capability, sterile workbench, fume hood, CO2 incubator and centrifuge