Overview of the P11 experimental hutch.
The macromolecular crystallography beamline P11 at Petra III is dedicated to diffraction experiments of biological samples. The beamline provides an end-station for crystallography experiment operating between 6 keV and 20 keV. While the optics setup of the beamline allows energies up to 30 keV, the stationary detector at P11 is not ideal for higher energy experiments and thus the experiments are limited to 20 keV. Higher energies with appropriate detector stationary at the beamline are readily available at other PETRA beamlines. Beamline P11 is dedicated to high-throughput macromolecular crystallography (MX) and aims to provide a stable and automated environment for this type experiments. Standard energy is 12 keV.
GENERAL SPECIFICATIONS
|
techniques available |
macromolecular crystallography (imaging of biological samples) |
|---|---|
|
photon source |
undulator |
|
period number of periods max power |
31.4 mm 63 9.5 kW |
|
source brilliance |
1018 ph / s / 0.1% bw / mA |
|
polarization available |
linear horizontal |
|
energy range |
5.5 keV - 30 keV |
|
beamline energy resolution |
1.3 x 10-4 (Si-111) / 0.28 x 10-4 (Si-113) |
|
max flux ON SAMPLE |
1.3 x 1013 ph/s at 12 keV |
|
spot size ON SAMPLE |
4 x 9 μm² up to 200 x 200 μm² (v x h) with full flux;
1 x 1 μm² with > 2 x 1011 ph/s |
|
angle of incidence light – sample |
0° to 60° |
|
sample type |
macromolecular crystals (biological tissue) |
OPTICS
|
type |
double crystal monochromator |
|---|---|
|
grating properties
|
Si-111 and Si-113 reflection |
|
energy range
|
2.4 keV - 30 keV (Si-111) |
|
energy resolution
|
~ 1 eV |
|
type |
1. horizontal focussing mirror |
|---|---|
|
material |
silicon |
|
gracing angle |
2.5 mrad |
|
coatings |
uncoated (2.4 keV - 8 keV) palladium (8 keV - 20 keV) platinum (> 20 keV) |
|
radius of curvature |
-∞ ≤ x ≤ -10 km; ∞ ≥ x ≥ +10 km |
|
type |
2. horizontal focussing mirror |
|---|---|
|
material |
silicon |
|
gracing angle |
2.5 mrad |
|
coatings |
uncoated (2.4 keV - 8 keV) palladium (8 keV - 20 keV) platinum (> 20 keV) |
|
radius of curvature |
-∞ ≤ x ≤ -10 km; ∞ ≥ x ≥ +10 km |
|
type |
vertical focussing mirror |
|---|---|
|
material |
silicon |
|
gracing angle |
2.5 mrad |
|
coatings |
uncoated (2.4 keV - 8 keV) palladium (8 keV - 20 keV) platinum (> 20 keV) |
|
radius of curvature |
100 km ≥ x ≥ 10 km |
ENDSTATION(S)
|
type |
High Precision 1-Axis Goniostat |
|---|---|
|
diffractometer |
inhouse-designed high precision goniometer; sphere of confusion: 100 nm |
|
manipulator |
single air-bearing-based rotation axis equipped with motor-driven crystal centering stages temperature range: 80 K - 350 K transfer system: manual / sample changing robot (uni-puck) control: fully automated |
|
detectors |
Eiger 2X 16M (replacing Pilatus 6M fast) Vortex-EM |
|
sample environment |
temperature range: 80 K - 400 K |
DETECTOR(S)
|
type |
Eiger 2X 16M |
|---|---|
|
count rate |
107 ph/s |
|
spatial resolution |
75 µm |
|
2theta range |
0° - 60° |
|
frame rate |
up to 100 Hz |
|
read-out |
continuous |
|
dead time between exposures |
< 100 ns (max.loss of 1 count/pixel) |
|
pixel size |
75 x 75 µm |
|
energy detection thresholds |
2 |
|
point-spread function |
1 pixel |
|
array size |
4148 x 4362 pixel |
|
field of view |
311.1 x 327.15 mm |
|
degrees of freedom / translation stages |
1 ; detector distance |
|
data format |
HDF5/NeXus |
|
typical collection time |
18 s per data set |
|
type |
Vortex-EM |
|---|---|
|
energy resolution |
100 eV |
|
count rate |
2 x 105 cts/s |
|
2theta range |
fixed 110° |
|
typical collection time |
1 s per energy point |
CONTROL/DATA ANALYSIS
|
Graphical user interface |
CrystalControl (in-house develeopment) |
|---|---|
|
Data output type |
diffraction images, fluorescence spectra |
|
Data output |
hdf5, tif, ascii, (cbf for Pilatus) |
|
software(s) for quantitative analysis |
XDS, XDSAPP (autoprocessing), CCP4, PHENIX, Mosflm (autoprocessing), Albula |
SUPPORT LABORATORY
P11 chemistry lab equipped with Leica 205C stereo microscope, crystal shifter, fume hood, ThermoStat, centrifuge, crystal growing incubator, and harvesting and cryo-cooling tools.
