Ultrafast lasers can protect a DNA building block from light-induced dissociation

ultrafast stabilisation of adenine against dissociation

Artist´s impression of the ultrafast stabilisation of adenine against dissociation: When the molecule is ionised by VUV radiation it undergoes dissociation, however, by taking advantage of a charge migration mechanism and by properly timing a second infrared laser pulse it is possible to stabilise it via a second ionisation event (image: U. De Giovannini MPSD).

An international research team led by DESY researcher Francesca Calegari and with the key participation of the Max Planck Institute for the Structure and Dynamics of Matter (MPSD) has demonstrated that ultrashort laser pulses can be used to protect one of the DNA building blocks against destruction induced by vacuum ultraviolet (VUV) radiation. The research group unveiled that a second laser flash in the infrared, timed shortly (only a few millionths of billions of a second) after the first VUV flash, prevented the adenine molecule to disintegrate, therefore stabilising it. The group presents their work in the journal Communications Chemistry published by Nature publishing group.

High energy radiation can cause irreparable damage to our own biological molecules – such as DNA – leading to mutations and potentially cell death. Damage is often occurring as a consequence of the molecular ionisation, inducing the fragmentation of the DNA subunits. So far, protection against radiation damage has hardly been achieved, as the photo-induced dissociation process could not be stopped. In their ultra-short-time experiments, Francesca Calegari´s research group and collaborators have discovered that, by taking advantage of mechanisms that take place on extremely fast time scales, it is indeed possible to protect the molecule.

Francesca Calegari, who is also Professor at Hamburg University, heads the attosecond research group at the Center for Free-Electron Laser Science (CFEL) which is jointly operated by DESY, the University of Hamburg and the Max Planck Society. She works with extremely short light flashes in the attosecond range, with the idea of imaging and controlling electron movements in complex molecules. In doing so, the group is particularly interested in investigating the role of electrons in photochemical processes, i.e. processes that are triggered by light.

In their recent experiments, the group now exposed molecules of the DNA building block adenine to an intense VUV flash for a time of attoseconds (10-18 s) which regularly causes the molecule to be destroyed. When they shone an infrared light flash on the molecule only two femtoseconds (10-15 s) afterwards, they found that the molecule stabilised itself by emitting an electron and its dissociation was stopped. Instead, a doubly ionised but otherwise intact adenine molecule remained. Overall, the group was thus able to save about one per cent of the molecules from destruction. The key mechanism behind this stabilisation has been found to be “electron charge migration”: a purely electronic process involving an ultrafast charge inflation away from the molecule. “In our experiments we have entered the attosecond range and were thus able to show - for the first time - that with our technology it is possible to take advantage of electron movements to change the fate of a molecular reaction,” explains first author Erik Månsson from DESY. “Until now, there have been only speculations about this possibility and the usual assumption was that controlling the movements of the atomic nuclei in the molecules was key for the molecular stabilisation." This study has been conducted over time scales in which the atoms in the molecule can be considered as frozen, therefore unveiling a purely electronic mechanism so far unexplored for the building blocks of DNA.

The new findings are an important step towards understanding the fastest mechanisms activated by the interaction of biomolecules with light and their role in photoprotection. “The demonstration of an ultrafast stabilisation protocol for a DNA subunit, by interrogating the system before the nuclear motion takes place, can substantially improve our ability of controlling ionisation damage effects – with interesting perspectives for protecting molecules against light,” explains Calegari.

(from DESY News)


Real-time observation of a correlation-driven sub 3 fs charge migration in ionised adenine; E. P. Månsson, S. Latini, F. Covito, V. Wanie, M. Galli, E. Perfetto, G. Stefanucci, H. Hübener, U. De Giovannini,M. C. Castrovilli, A. Trabattoni, F. Frassetto, L. Poletto, J. B. Greenwood, F. M. Nisoli, A.l Rubioand F. Calegari; Communications Chemistry, 2021; DOI: 10.1038/s42004-021-00510-5