Unified Data Sheet

Overview of the P11 experimental hutch.

P11 - The Bio-Imaging and Diffraction Beamline

The Bio-imaging and diffraction beamline P11 at Petra III is dedicated to imaging and diffraction experiments of biological samples. The beamline provides two different endstations: A crystallography experiment operating between 6 keV and 30 keV and an X-ray microscope utilizing tender X-rays between 2.4 and 10 keV. The basic idea for the beamline design is to provide an extremely stable and flexible setup ideally suited for micro- and nano-beam applications.

CONTACT

GENERAL SPECIFICATIONS

techniques available

macromolecular crystallography (imaging of biological samples)

photon source

undulator

period

number of periods

max power

31.4 mm

63

9.5 kW

source brilliance 

1018 ph / s / 0.1% bw / mA

polarization available

linear horizontal

energy range 

  5.5 keV - 30 keV

beamline energy resolution

1.3 x 10-4 (Si-111) / 0.28 x 10-4 (Si-113)

max flux ON SAMPLE

1.3 x 1013 ph/s at 12 keV

spot size ON SAMPLE 

  4 x 9 μm² up to 300 x 300 μm²  (v x h)  with full flux;
  1 x 1 μwith > 2 x 1011 ph/s

angle of incidence light – sample 

0° to 60°

sample type

macromolecular crystals (biological tissue)

OPTICS

type

  double crystal monochromator

grating properties 

Si-111 and Si-113 reflection

energy range         

2.4 keV - 30 keV (Si-111)

resolving power 

~ 1 eV

type

  1. horizontal focussing mirror

 material

silicon

gracing angle

2.5 mrad

 coatings      

uncoated (2.4 keV - 8 keV)

palladium (8 keV - 20 keV)

platinum (> 20 keV)

 radius of curvature 

-10 km - +10 km

type

  2. horizontal focussing mirror

 material

silicon

gracing angle

2.5 mrad

 coatings      

uncoated (2.4 keV - 8 keV)

palladium (8 keV - 20 keV)

platinum (> 20 keV)

 radius of curvature 

-10 km - +10 km

type

  vertical focussing mirror

 material

silicon

gracing angle

2.5 mrad

 coatings      

uncoated (2.4 keV - 8 keV)

palladium (8 keV - 20 keV)

platinum (> 20 keV)

 radius of curvature 

100 km - 10 km

ENDSTATION(S)

High Precision 1-Axis Goniostat

diffractometer

inhouse-designed high precision goniometer

manipulator

single air-bearing-based rotation axis equipped with piezomotor-driven crystal centering stages

temperature range: 80 K - 350 K

transfer system: manual / sample changing robot (uni-puck)

control: fully automated


detectors

Pilatus 6M fast

Vortex-EM

sample environment

temperature range: 80 K - 400 K

DETECTOR(S)

Area Detector(s)

type 

Pilatus 6M fast

count rate 

 106 ph/s

spatial resolution 

  172 µm

2theta range  

  0° - 60°

frame rate

up to 25 Hz

read-out time 

  2.6 ms (continuous data collection)

pixel size         

172 µm

array size 

  2463 x 2527 pixel

field of view  

423.6 x 434.6 mm2

 degrees of freedom / translation stages

1 - detector distance

 typical collection time

90 s per data set

Point Detector(s)

type 

Vortex-EM

energy resolution 

100 eV

count rate 

2 x 105 cts/s

2theta range  

fixed 110°

 typical collection time

1 s per energy point

CONTROL/DATA ANALYSIs

software type

custom-designed graphical user interface (P11 GUI)

data output type

diffraction images, fluorescence spectra

data output

cbf, tif, ascii

software(s) for quantitative analysis

XDS, PHENIX, Mosflm, CCP4, COOT, Xia2 (for automatic data processing)

SUPPORT LAB

wet lab equipped with Leica 205C stereo microscope, Nikon fluorescence microscope with cryo-cooling capability, sterile workbench, fume hood, autoclave, CO2 incubator and centrifuge